Prevalence of MRSA Colonization in 150 Neonates in two Medical
Centers in Iran.
Professor of Pediatrics, ID ,
Shahid Beheshti University of Medical Science ,
Dr A. H. Arbabi,MD
Assistant prof.of Iran University ,Tehran, Iran.
Dr F. Shirvani , MD,
ID ,Assistant Prof.of Pediatrics Shahid Beheshti University , Tehran
Dr K. Mirnia,
MD,Resident .of Pediatrics, Shahid Beheshti University,Tehran, Iran.
Dr. M. Radfar, MD,
Assistant Prof. Of Pediatrics .Neonatologist,Shahid Beheshti
Dr. M. Khodami , MD ,
Associate of Pathology ,Shhid Beheshti University,Tehran, Iran.
M. Gharouni .Microbiologist,Shahid
Penicillin was introduced for s- aureus treatment in 1940.
However with the over usage this antibiotic, S- aureus has become
increasingly Antibiotic Resistant .
Due to this problem we studied the
epidemiological prevalence of MRSA colonization on 150 Neonates in
two Medical Centers, Imam Hossein and Shahid Akbarabadi in
Methods : Skin and
Umbilical samples obtained from 150 newborns in two Medical Centers
after 72 hours of admission. Also samples were obtained from
anterior nasal mucosa of personnel involved with Neonates in that
department by sterile cotton swap .
Sterile swap was inoculated in to nutrient
agar then within 24 h was transferred to sheep blood agar. Colonies
that were gram positive in smear and were coagulase positive and
DNAase positive were known as s-aureus . s-aureus were transformed
to mulleur hinton NaCl 4 % culture that had Oxacillin Disk.
After 24 h of incubation at 37° C , colonies
that grew <13 mm around the Disk were known as MRSA , and colonies
that grew > 13 mm were known as MSSA .
Information related to neonates and personnel
were collected by completing a questionnaire. All the data were
analyzed by statistical software SPSS-11.
In our study, 47/3 % of neonates were Male and 52/7 %
were female . Prevalence of MRSA in male was 52/8 % and in female
47/2 %. Mean weight of newborns were 2394 grams +/- 1119 grams.
61 % of S- aureus were Resistant to Oxacillin.
Prevalence of MRSA in Imam Hossain Hospital was 96/6 % and in Shahid
Akbarabadi was 26/7 % that this difference was statistically
significant P < 0.0001. All MRSA were sensitive to Vancomycin in
Imam Hossain Hospital. Difference of MRSA S-resistant to
Cloxacillin and Clindamycin were statistically significant P
In Akbarabadi Hospital difference of MRSA
S-Resistant to Cloxacillin. Clindamycin and Penicillin were not
Hospital and Mothers were the most effective
agent in MRSA S Prevalence.
Hossein is a general Hospital including an adult Surgical Ward but
Shahid Akbarabadi Hospital is a Delivery Center that has an active
NICU . Prevalence of MRSA in Imam Hossein Hospital is greater than
Shahid Akbarabadi Hospital due to more variety of patients and over
usage Antibiotics. MRSA colonies resistant to Cloxacillin ,
Clindamycin and Penicillin are more in Immam Hossain Hospital and
therefore these antibiotics are not used. This Resistance is fewer
in Shahid Akbarabadi Hospital and therefore these Antibiotics are